Hotspot

psbE-ORF31

LSC · 63912-65008 · 1097 bp

Back to Bunias

Pi 0.0253
MarkerSeek score 60.30
Primer available yes
Label psbE-ORF31

Feature Metadata

label_name
psbE-ORF31
feature_type
igs
region
LSC
length_bp
1097
variable_sites
20
pi
0.0253
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.72
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-ORF31_p1 ATAACCAGCCCGCAATGAAT AGCTGCTAGTAGAAAACCGA 45.0 45.0 57.9 56.3 1235-1248 bp 1.0 71.0
psbE-ORF31_p2 AATAACCAGCCCGCAATGAA AGCTGCTAGTAGAAAACCGA 45.0 45.0 58.2 56.3 1236-1249 bp 1.0 70.4
psbE-ORF31_p3 AGAACGTTCTCCTGTGCTTC AGCTGCTAGTAGAAAACCGA 50.0 45.0 57.8 56.3 1153-1166 bp 1.0 70.4
psbE-ORF31_p4 GTTCTCCTGTGCTTCCAGAC AGCTGCTAGTAGAAAACCGA 55.0 45.0 58.2 56.3 1148-1161 bp 1.0 70.1
psbE-ORF31_p5 ATAACCAGCCCGCAATGAAT AGCTGCTAGTAGAAAACCGAA 45.0 42.9 57.9 57.0 1235-1248 bp 1.0 69.1