Hotspot

trnF-ndhJ

LSC · 47684-48262 · 579 bp

Back to Bunias

Pi 0.0295
MarkerSeek score 70.60
Primer available yes
Label trnF-ndhJ

Feature Metadata

label_name
trnF-ndhJ
feature_type
igs
region
LSC
length_bp
579
variable_sites
17
pi
0.0295
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-ndhJ_p1 CCTCGTGTCACCAGTTCAAA CATCCACGACTGAAACGGAT 50.0 50.0 58.1 58.0 718-724 bp 1.0 81.6
trnF-ndhJ_p2 CCTCGTGTCACCAGTTCAAA CCCGAAAGTTGGATAGGGTG 50.0 55.0 58.1 58.0 691-697 bp 1.0 81.5
trnF-ndhJ_p3 CCTCGTGTCACCAGTTCAAA CCGAAAGTTGGATAGGGTGG 50.0 55.0 58.1 58.0 690-696 bp 1.0 81.5
trnF-ndhJ_p4 CCTCGTGTCACCAGTTCAAA CCACGACTGAAACGGATCTT 50.0 50.0 58.1 57.9 715-721 bp 1.0 81.1
trnF-ndhJ_p5 CCTCGTGTCACCAGTTCAAA TGATAGCCATCCACGACTGA 50.0 50.0 58.1 58.2 725-731 bp 1.0 79.9