Hotspot

trnfM-rps14

LSC · 36405-36562 · 158 bp

Back to Bunias

Pi 0.038
MarkerSeek score 69.80
Primer available yes
Label trnfM-rps14

Feature Metadata

label_name
trnfM-rps14
feature_type
igs
region
LSC
length_bp
158
variable_sites
6
pi
0.038
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-rps14_p1 GCTACCAAACTGCTCTACCC GGGAAATGGTTCAGGCATGT 55.0 50.0 58.0 58.4 235 bp 1.0 78.3
trnfM-rps14_p2 AAGGTTATGAGCCTTGCGAG GGGAAATGGTTCAGGCATGT 50.0 50.0 58.0 58.4 254 bp 1.0 78.1
trnfM-rps14_p3 TTTGAACCCGTGACCTCAAG GGGAAATGGTTCAGGCATGT 50.0 50.0 58.0 58.4 271 bp 1.0 77.9
trnfM-rps14_p4 GCTACCAAACTGCTCTACCC TCCTTCGGGAAATGGTTCAG 55.0 50.0 58.0 57.5 241 bp 1.0 77.9
trnfM-rps14_p5 AAGGTTATGAGCCTTGCGAG TCCTTCGGGAAATGGTTCAG 50.0 50.0 58.0 57.5 260 bp 1.0 77.7