Hotspot

rpoB-trnC

LSC · 26102-27079 · 978 bp

Back to Bunias

Pi 0.0297
MarkerSeek score 67.40
Primer available yes
Label rpoB-trnC

Feature Metadata

label_name
rpoB-trnC
feature_type
igs
region
LSC
length_bp
978
variable_sites
29
pi
0.0297
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC_p1 GTTCCCTCTTTTCCATCCCC CCGGATTTGAACTGGGGAAA 55.0 50.0 57.9 58.1 1066 bp 1.0 80.6
rpoB-trnC_p2 GTTCCCTCTTTTCCATCCCC AAAAGGATTTGCAGTCCCCC 55.0 50.0 57.9 58.4 1048 bp 1.0 78.4
rpoB-trnC_p3 TGTTCCCTCTTTTCCATCCC CCGGATTTGAACTGGGGAAA 50.0 50.0 57.1 58.1 1067 bp 1.0 74.5
rpoB-trnC_p4 GTTCCCTCTTTTCCATCCCC AAGTCTGTTGTTGAGGCGG 55.0 52.6 57.9 58.0 1088 bp 1.0 73.1
rpoB-trnC_p5 TCTTTTCCATCCCCGAGCAT CCGGATTTGAACTGGGGAAA 50.0 50.0 59.1 58.1 1060 bp 1.0 73.1