Pi
0.0186
MarkerSeek score
69.80
Primer available
yes
Label
atpH-atpI
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| atpH-atpI_p1 | AACCGAAGCAGCAGAAATCA | TACAAGCGGGATTCAAGCTC | 45.0 | 50.0 | 58.1 | 58.0 | 594-607 bp | 1.0 | 81.9 |
| atpH-atpI_p2 | TACCTTGACCAACTCCAGGT | TACAAGCGGGATTCAAGCTC | 50.0 | 50.0 | 57.9 | 58.0 | 652-665 bp | 1.0 | 81.6 |
| atpH-atpI_p3 | GTCCAATAGAAGCAAGCCCA | TACAAGCGGGATTCAAGCTC | 50.0 | 50.0 | 57.9 | 58.0 | 634-647 bp | 1.0 | 81.5 |
| atpH-atpI_p4 | GCTGTACCTTGACCAACTCC | TACAAGCGGGATTCAAGCTC | 55.0 | 50.0 | 58.2 | 58.0 | 656-669 bp | 1.0 | 80.9 |
| atpH-atpI_p5 | AACCGAAGCAGCAGAAATCA | GCGGGATTCAAGCTCTCATT | 45.0 | 50.0 | 58.1 | 58.3 | 589-602 bp | 1.0 | 79.5 |