Hotspot

psbI-trnS

LSC · 7612-7708 · 97 bp

Back to Bunias

Pi 0.0667
MarkerSeek score 76.20
Primer available yes
Label psbI-trnS

Feature Metadata

label_name
psbI-trnS
feature_type
igs
region
LSC
length_bp
97
variable_sites
6
pi
0.0667
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS_p1 TCCTATCTAATGATCCAGGACGT TAGGAGAGATGGCTGAGTGG 43.5 55.0 58.0 57.9 225-231 bp 1.0 59.9
psbI-trnS_p2 TCCTATCTAATGATCCAGGACGT TGAGTGGACTAAAGCGTTGG 43.5 50.0 58.0 57.8 212-218 bp 1.0 59.5
psbI-trnS_p3 TCCTATCTAATGATCCAGGACGT GCTGAGTGGACTAAAGCGTT 43.5 50.0 58.0 58.2 214-220 bp 1.0 59.2
psbI-trnS_p4 TCCTATCTAATGATCCAGGACGT CGAGTTAATCGTACCGAGGG 43.5 55.0 58.0 57.6 175-181 bp 1.0 58.7
psbI-trnS_p5 TCCTATCTAATGATCCAGGACGT ATGGCTGAGTGGACTAAAGC 43.5 50.0 58.0 57.6 217-223 bp 1.0 57.4