Hotspot

psbK-psbI

LSC · 7106-7500 · 395 bp

Back to Bunias

Pi 0.0329
MarkerSeek score 62.40
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
395
variable_sites
13
pi
0.0329
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCAGCTGTAAG ACAAAAAGTTTGAGAGTAAGCA 50.0 31.8 57.9 54.4 448-475 bp 1.0 42.3
psbK-psbI_p2 TGGCAAGCAGCTGTAAGTTT ACAAAAAGTTTGAGAGTAAGCA 45.0 31.8 58.3 54.4 445-472 bp 1.0 42.3
psbK-psbI_p3 TTGGCAAGCAGCTGTAAGTT ACAAAAAGTTTGAGAGTAAGCA 45.0 31.8 58.3 54.4 446-473 bp 1.0 42.3
psbK-psbI_p4 TTTGGCAAGCAGCTGTAAGT ACAAAAAGTTTGAGAGTAAGCA 45.0 31.8 58.3 54.4 447-474 bp 1.0 42.3
psbK-psbI_p5 GTTTGGCAAGCAGCTGTAAG ACAAAAAGTTTGAGAGTAAGCAT 50.0 30.4 57.9 55.0 448-475 bp 1.0 42.3