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clpP-psbB

LSC · 73373-74055 · 683 bp

Back to Bulbophyllum

Pi 0.0518

MarkerSeek score 86.90

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 683
variable_sites: 136
pi: 0.0518
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 86.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	714-830 bp	1.0	90.1
clpP-psbB_p2	TATACGTCAACCCAAGCTGC	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	709-825 bp	1.0	90.0
clpP-psbB_p3	CTTTTGGAACACCAATGGGC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	660-776 bp	1.0	88.8
clpP-psbB_p4	CGCACTATACGTCAACCCAA	ACACGATACCAAGGCAAACC	50.0	50.0	58.0	58.5	717-833 bp	1.0	88.2
clpP-psbB_p5	TATACGTCAACCCAAGCTGC	ACACGATACCAAGGCAAACC	50.0	50.0	58.0	58.5	712-828 bp	1.0	88.1