Hotspot

psaJ-rpl33

LSC · 68201-68808 · 608 bp

Back to Bulbophyllum

Pi 0.052

MarkerSeek score 82.40

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 608
variable_sites: 133
pi: 0.052
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TACCAAACTGCGCTACATCC	AATGCCCGGCGATTCTTTAT	50.0	45.0	58.0	58.0	1027-1270 bp	1.0	91.7
psaJ-rpl33_p2	TACCAAACTGCGCTACATCC	ATGCCCGGCGATTCTTTATT	50.0	45.0	58.0	58.0	1026-1269 bp	1.0	91.7
psaJ-rpl33_p3	TTTTACCCCCGAGGAGTCTT	AATGCCCGGCGATTCTTTAT	50.0	45.0	58.0	58.0	1259-1438 bp	0.736842	81.0
psaJ-rpl33_p4	TTTTACCCCCGAGGAGTCTT	ATGCCCGGCGATTCTTTATT	50.0	45.0	58.0	58.0	1258-1437 bp	0.736842	81.0
psaJ-rpl33_p5	TTTTTACCCCCGAGGAGTCT	ATGCCCGGCGATTCTTTATT	50.0	45.0	58.0	58.0	1259-1438 bp	0.736842	81.0