Hotspot

petN-psbM

LSC · 29439-30194 · 756 bp

Back to Bulbophyllum

Pi 0.0422

MarkerSeek score 81.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 756
variable_sites: 164
pi: 0.0422
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	CTTGCGCTTATTGCTACTGC	50.0	50.0	58.2	57.9	837-1010 bp	0.947368	87.9
petN-psbM_p2	GTGGCTCAAACAGGTACCAA	CTTGCGCTTATTGCTACTGC	50.0	50.0	58.0	57.9	1428-1608 bp	0.842105	84.7
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	ACGCCCCTTTTTGAAGGAAT	50.0	45.0	58.2	58.0	1328-1532 bp	0.842105	84.4
petN-psbM_p4	GTGGCTCAAACAGGTACCAA	AACGCCCCTTTTTGAAGGAA	50.0	45.0	58.0	58.2	1932-2116 bp	0.842105	83.8
petN-psbM_p5	GTGGCTCAAACAGGTACCAA	ACGCCCCTTTTTGAAGGAAT	50.0	45.0	58.0	58.0	1931-2115 bp	0.789474	83.3