Hotspot

matK-trnK-UUU

LSC · 3273-4341 · 1069 bp

Back to Bulbophyllum

Pi 0.0361

MarkerSeek score 77.90

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 1069
variable_sites: 189
pi: 0.0361
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AATTCTCGTGCCCCCAAAAT	AACGGTAGAGTACTCGGCTT	45.0	50.0	58.1	58.2	1275-1422 bp	0.789474	81.7
matK-trnK-UUU_p2	AATTCTCGTGCCCCCAAAAT	ACGGTAGAGTACTCGGCTTT	45.0	50.0	58.1	58.2	1274-1421 bp	0.789474	81.7
matK-trnK-UUU_p3	ATTCTCGTGCCCCCAAAATT	AACGGTAGAGTACTCGGCTT	45.0	50.0	58.1	58.2	1274-1421 bp	0.736842	79.6
matK-trnK-UUU_p4	ATTCTCGTGCCCCCAAAATT	ACGGTAGAGTACTCGGCTTT	45.0	50.0	58.1	58.2	1273-1420 bp	0.736842	79.6
matK-trnK-UUU_p5	ATTCTCGTGCCCCCAAAATT	GTGCTAAACCCACAGGAACT	45.0	50.0	58.1	57.7	1903-2068 bp	0.736842	78.8