Hotspot

ycf1

SSC · 124067-129472 · 5406 bp

Back to Buddleja

Pi 0.0105

MarkerSeek score 54.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5406
variable_sites: 355
pi: 0.0105
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTTTATGTAGCGAAATGGTC	ACAGCAGAAGATCGTTCTCC	33.3	50.0	52.5	57.3	178 bp	1.0	48.0
ycf1_p2	TTTTTATGTAGCGAAATGGTC	AGGGTGGAAAGTGAGGAAGA	33.3	50.0	52.5	57.9	217 bp	0.678571	35.1
ycf1_p3	TTTTTATGTAGCGAAATGGTC	ACGGAAGAAAGGGTGGAAAG	33.3	50.0	52.5	57.7	226 bp	0.678571	35.1
ycf1_p4	TTTTTATGTAGCGAAATGGTC	CGGAAGAAAGGGTGGAAAGT	33.3	50.0	52.5	57.7	225 bp	0.678571	35.1
ycf1_p5	TTTTTATGTAGCGAAATGGTC	AACGGAAGAAAGGGTGGAAA	33.3	45.0	52.5	57.3	227 bp	0.678571	35.1