Hotspot

ccsA-ndhD

SSC · 115595-115834 · 240 bp

Back to Buddleja

Pi 0.0209

MarkerSeek score 69.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 240
variable_sites: 30
pi: 0.0209
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTCAGCAATTGTGGCGTCTA	ACCCCGATTTCGTTCTTTCA	45.0	45.0	57.8	57.4	409-430 bp	1.0	84.4
ccsA-ndhD_p2	GCAATTGTGGCGTCTATTGG	ACCCCGATTTCGTTCTTTCA	50.0	45.0	57.8	57.4	405-426 bp	1.0	84.3
ccsA-ndhD_p3	AATTCAGCAATTGTGGCGTC	ACCCCGATTTCGTTCTTTCA	45.0	45.0	57.6	57.4	411-432 bp	1.0	82.9
ccsA-ndhD_p4	TCAGCAATTGTGGCGTCTAT	ACCCCGATTTCGTTCTTTCA	45.0	45.0	57.6	57.4	408-429 bp	1.0	82.6
ccsA-ndhD_p5	ATTCAGCAATTGTGGCGTCT	ACCCCGATTTCGTTCTTTCA	45.0	45.0	58.5	57.4	410-431 bp	1.0	82.1