Hotspot

rpl32-trnL-UAG

SSC · 113550-114447 · 898 bp

Back to Buddleja

Pi 0.0203

MarkerSeek score 71.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 898
variable_sites: 112
pi: 0.0203
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GTGCAACAAACAAATAAAATAAGT	TCCTAAGAGCAGCGTGTCTA	25.0	50.0	53.6	57.9	951-998 bp	0.964286	49.3
rpl32-trnL-UAG_p2	GTGCAACAAACAAATAAAATAAGT	AGCAGCGTGTCTACCAATTT	25.0	45.0	53.6	57.8	944-991 bp	0.964286	49.3
rpl32-trnL-UAG_p3	GTGCAACAAACAAATAAAATAAGT	CCTAAGAGCAGCGTGTCTAC	25.0	55.0	53.6	57.8	950-997 bp	0.964286	49.3
rpl32-trnL-UAG_p4	GTGCAACAAACAAATAAAATAAGT	CTAAGAGCAGCGTGTCTACC	25.0	55.0	53.6	57.8	949-996 bp	0.964286	49.3
rpl32-trnL-UAG_p5	GTGCAACAAACAAATAAAATAAGT	TTTAGGAGATGACATGCCGC	25.0	50.0	53.6	58.0	1006-1053 bp	0.964286	49.1