Hotspot

ndhF-rpl32

SSC · 112867-113372 · 506 bp

Back to Buddleja

Pi 0.0157
MarkerSeek score 65.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
506
variable_sites
50
pi
0.0157
unique_haplotype_count
22
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAAGGTATGATCCACGCA TGATGCAGAAGTACGTTTTT 50.0 35.0 57.7 53.5 569-594 bp 1.0 51.9
ndhF-rpl32_p2 GGAAAGGTATGATCCACGCA TTGATGCAGAAGTACGTTTT 50.0 35.0 57.7 53.5 570-595 bp 1.0 51.9
ndhF-rpl32_p3 GGAAAGGTATGATCCACGCA TTTGATGCAGAAGTACGTTT 50.0 35.0 57.7 53.5 571-596 bp 1.0 51.9
ndhF-rpl32_p4 GGAAAGGTATGATCCACGCA TTTTGATGCAGAAGTACGTT 50.0 35.0 57.7 53.5 572-597 bp 1.0 51.9
ndhF-rpl32_p5 GGAAAGGTATGATCCACGCA TTTTTGATGCAGAAGTACGT 50.0 35.0 57.7 53.5 573-598 bp 1.0 51.9