Hotspot

petA-psbJ

LSC · 64442-65375 · 934 bp

Back to Buddleja

Pi 0.00918

MarkerSeek score 69.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 934
variable_sites: 57
pi: 0.00918
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.2	58.1	1040-1136 bp	1.0	77.8
petA-psbJ_p2	TCGAGAAGGTTCAATTGGCC	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.2	58.1	1042-1138 bp	1.0	77.8
petA-psbJ_p3	GAGAAGGTTCAATTGGCCGA	AGCTGGTATTCTTGTGATCGG	50.0	47.6	58.2	57.8	1041-1137 bp	1.0	77.0
petA-psbJ_p4	TCGAGAAGGTTCAATTGGCC	AGCTGGTATTCTTGTGATCGG	50.0	47.6	58.2	57.8	1043-1139 bp	1.0	77.0
petA-psbJ_p5	GATCCATTACGGGTCCAAGG	GCTGGTATTCTTGTGATCGGT	55.0	47.6	57.7	58.1	1127-1223 bp	1.0	76.7