Hotspot

ycf4-cemA

LSC · 61707-62580 · 874 bp

Back to Buddleja

Pi 0.00754

MarkerSeek score 67.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 874
variable_sites: 42
pi: 0.00754
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	ATTCCCTTGACCCGTACTGA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	916-1056 bp	1.0	86.4
ycf4-cemA_p2	TTCCCTTGACCCGTACTGAT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	915-1055 bp	1.0	86.4
ycf4-cemA_p3	TATTTCTTGCGCGTACCGAT	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	847-987 bp	1.0	85.2
ycf4-cemA_p4	GAACAAAAAGCCGCGGAATT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.2	58.1	871-1011 bp	1.0	85.2
ycf4-cemA_p5	GCTATTCCCTTGACCCGTAC	GAGAAATCCACCAGGGCAAA	55.0	50.0	57.8	58.1	919-1059 bp	1.0	84.9