Hotspot

ndhC-trnV-UAC

LSC · 51360-52510 · 1151 bp

Back to Buddleja

Pi 0.0102

MarkerSeek score 72.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1151
variable_sites: 78
pi: 0.0102
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAATCCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1272-1288 bp	0.964286	83.2
ndhC-trnV-UAC_p2	GGGGCTAAAATCCCGGAAAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	1260-1281 bp	1.0	81.2
ndhC-trnV-UAC_p3	TTCTCCGGCCCTTTCCTAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	1293-1309 bp	0.964286	80.4
ndhC-trnV-UAC_p4	GGGGCTAAAATCCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1271-1287 bp	0.964286	80.4
ndhC-trnV-UAC_p5	CCCTTTCCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.6	57.8	1285-1301 bp	0.964286	79.2