Hotspot

ycf3-trnS-GGA

LSC · 45088-45918 · 831 bp

Back to Buddleja

Pi 0.0074

MarkerSeek score 61.80

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 831
variable_sites: 44
pi: 0.0074
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TGCCTCCTTTTCTCCTGAAG	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	993-1026 bp	1.0	76.8
ycf3-trnS-GGA_p2	GCCTCCTTTTCTCCTGAAGT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	992-1025 bp	1.0	76.8
ycf3-trnS-GGA_p3	TGCCTCCTTTTCTCCTGAAG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.1	58.2	1014-1047 bp	1.0	76.2
ycf3-trnS-GGA_p4	GCCTCCTTTTCTCCTGAAGT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.1	58.2	1013-1046 bp	1.0	76.2
ycf3-trnS-GGA_p5	TGCCTCCTTTTCTCCTGAAG	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.1	57.8	964-997 bp	1.0	76.1