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psbZ-trnG-GCC

LSC · 36715-37018 · 304 bp

Back to Buddleja

Pi 0.0161

MarkerSeek score 63.20

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 304
variable_sites: 31
pi: 0.0161
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	AATCGAACCCGCATCTTCTC	50.0	50.0	57.8	58.1	447-479 bp	1.0	87.6
psbZ-trnG-GCC_p2	TTGCTTCTCCTGATGGTTGG	GAATCGAACCCGCATCTTCT	50.0	50.0	57.8	58.1	448-480 bp	1.0	87.5
psbZ-trnG-GCC_p3	GCTTCTCCTGATGGTTGGTC	AATCGAACCCGCATCTTCTC	55.0	50.0	58.3	58.1	445-477 bp	1.0	87.2
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	GAATCGAACCCGCATCTTCT	55.0	50.0	58.3	58.1	446-478 bp	1.0	87.2
psbZ-trnG-GCC_p5	CCGTCGTATTTGCTTCTCCT	AATCGAACCCGCATCTTCTC	50.0	50.0	57.7	58.1	456-488 bp	1.0	86.7