Hotspot

trnC-GCA-petN

LSC · 28389-29229 · 841 bp

Back to Buddleja

Pi 0.0126
MarkerSeek score 68.60
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
841
variable_sites
62
pi
0.0126
unique_haplotype_count
21
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG ACTTCTTCCCCACACTACGA 50.0 50.0 58.1 58.0 913-956 bp 1.0 88.5
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG TTCTTCCCCACACTACGAGT 50.0 50.0 58.1 58.0 911-954 bp 1.0 88.5
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG CACTTCTTCCCCACACTACG 50.0 55.0 58.1 57.9 914-957 bp 1.0 87.9
trnC-GCA-petN_p4 TTGTATCAGTTTGGCGGCAT ACTTCTTCCCCACACTACGA 45.0 50.0 58.2 58.0 967-1010 bp 1.0 87.7
trnC-GCA-petN_p5 TTGTATCAGTTTGGCGGCAT TTCTTCCCCACACTACGAGT 45.0 50.0 58.2 58.0 965-1008 bp 1.0 87.7