Hotspot

trnK-UUU-rps16

LSC · 4205-5094 · 890 bp

Back to Buddleja

Pi 0.0155
MarkerSeek score 70.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
890
variable_sites
82
pi
0.0155
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GGAACTTCCGTCTAATCAAACG 50.0 45.5 58.2 57.4 944-974 bp 1.0 69.0
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GGAACTTCCGTCTAATCAAACG 50.0 45.5 58.2 57.4 943-973 bp 1.0 69.0
trnK-UUU-rps16_p3 TCTAGCCGCACTTAAAAGCC GGAACTTCCGTCTAATCAAACG 50.0 45.5 58.3 57.4 958-988 bp 1.0 68.2
trnK-UUU-rps16_p4 TCAGTCGTGGTCTTCCAAAC GGAACTTCCGTCTAATCAAACG 50.0 45.5 57.8 57.4 1029-1059 bp 1.0 67.8
trnK-UUU-rps16_p5 CAGTCGTGGTCTTCCAAACT GGAACTTCCGTCTAATCAAACG 50.0 45.5 57.8 57.4 1028-1058 bp 1.0 67.7