Hotspot

ccsA

Genome · 66581-67594 · 1014 bp

Back to Bryopsis

Pi 0.106

MarkerSeek score 82.00

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: Genome
length_bp: 1014
variable_sites: 104
pi: 0.106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CAACACAATCCACACCTCCA	ACACGTTGTTTCTGACCTCC	50.0	50.0	58.0	58.1	2173 bp	0.5	59.4
ccsA_p2	CAACACAATCCACACCTCCA	CACGTTGTTTCTGACCTCCA	50.0	50.0	58.0	58.1	2172 bp	0.5	59.4
ccsA_p3	GACCTCCTGCTTCAACACAA	ACACGTTGTTTCTGACCTCC	50.0	50.0	58.0	58.1	2185 bp	0.5	59.3
ccsA_p4	GACCTCCTGCTTCAACACAA	CACGTTGTTTCTGACCTCCA	50.0	50.0	58.0	58.1	2184 bp	0.5	59.3
ccsA_p5	TGGCCAGTTCGACTTTCAAA	ACACGTTGTTTCTGACCTCC	45.0	50.0	58.0	58.1	1601 bp	0.5	59.2