Hotspot

trnR-rpoB

Genome · 132938-145188 · 12251 bp

Back to Bryopsis

Pi 0.43

MarkerSeek score 64.90

Primer available yes

Label trnR-rpoB

Feature Metadata

label_name: trnR-rpoB
feature_type: igs
region: Genome
length_bp: 12251
variable_sites: 1967
pi: 0.43
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.37
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-rpoB_p1	TTTCGATTGAGTGAGCTCCG	GAATGTGGCCGAAACTTTGG	50.0	50.0	58.0	57.9	662 bp	0.5	59.5
trnR-rpoB_p2	TTTCGATTGAGTGAGCTCCG	AACGTCTTGCTTTGCCAAAC	50.0	45.0	58.0	58.1	712 bp	0.5	59.3
trnR-rpoB_p3	TTTCGATTGAGTGAGCTCCG	CTTGCTTTGCCAAACACGAA	50.0	45.0	58.0	58.1	707 bp	0.5	59.3
trnR-rpoB_p4	GTTTGATAGGGTCACCGGTC	GAATGTGGCCGAAACTTTGG	55.0	50.0	58.0	57.9	396 bp	0.5	59.3
trnR-rpoB_p5	GTGGAAAGTTTCGGGGAGTT	GAATGTGGCCGAAACTTTGG	50.0	50.0	58.0	57.9	559 bp	0.5	59.3