Hotspot

trnS-psaJ

Genome · 99051-120265 · 21215 bp

Back to Bryopsis

Pi 0.441

MarkerSeek score 67.60

Primer available yes

Label trnS-psaJ

Feature Metadata

label_name: trnS-psaJ
feature_type: igs
region: Genome
length_bp: 21215
variable_sites: 5666
pi: 0.441
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.61
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-psaJ_p1	TTTAGGCGCCTCTAACGAAC	GGATCTCTCTCTTCCACGGA	50.0	55.0	58.0	57.9	536 bp	0.5	59.5
trnS-psaJ_p2	TTTAGGCGCCTCTAACGAAC	AGGCTGGGATCTCTCTCTTC	50.0	55.0	58.0	57.9	542 bp	0.5	59.3
trnS-psaJ_p3	TAGGCGCCTCTAACGAACTA	GGATCTCTCTCTTCCACGGA	50.0	55.0	58.0	57.9	534 bp	0.5	59.2
trnS-psaJ_p4	GCTCCCGGATCTTGTTGATT	GGATCTCTCTCTTCCACGGA	50.0	55.0	58.0	57.9	964 bp	0.5	59.2
trnS-psaJ_p5	TAGGCGCCTCTAACGAACTA	AGGCTGGGATCTCTCTCTTC	50.0	55.0	58.0	57.9	540 bp	0.5	59.0