Hotspot

psbT-psbH

Genome · 71267-71767 · 501 bp

Back to Bryopsis

Pi 0.101

MarkerSeek score 79.60

Primer available yes

Label psbT-psbH

Feature Metadata

label_name: psbT-psbH
feature_type: igs
region: Genome
length_bp: 501
variable_sites: 49
pi: 0.101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbH_p1	GATATCCCATTCCGACGAGC	GATTATGAGCCTTGCGAGCT	55.0	50.0	58.0	58.1	1617-1633 bp	1.0	86.7
psbT-psbH_p2	GATATCCCATTCCGACGAGC	AATGGATCACGGAGTTGCTC	55.0	50.0	58.0	58.0	1811 bp	0.5	59.7
psbT-psbH_p3	TGACGACGGCATTGTAAGAG	AATGGATCACGGAGTTGCTC	50.0	50.0	58.0	58.0	1833 bp	0.5	59.7
psbT-psbH_p4	GACGGCATTGTAAGAGCTGA	AATGGATCACGGAGTTGCTC	50.0	50.0	58.0	58.0	1829 bp	0.5	59.7
psbT-psbH_p5	GCTGTTGGTTGGTTAGGTCA	AATGGATCACGGAGTTGCTC	50.0	50.0	58.0	58.0	1940 bp	0.5	59.6