Hotspot

rpl36-infA

Genome · 39942-39995 · 54 bp

Back to Bryopsis

Pi 0.18

MarkerSeek score 79.80

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: Genome
length_bp: 54
variable_sites: 9
pi: 0.18
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	ACAGAGGCACGTATTTTCAT	TATGATTTAACACGTGGGCG	40.0	45.0	55.1	56.2	121-125 bp	1.0	50.9
rpl36-infA_p2	ACAGAGGCACGTATTTTCAT	ATATGATTTAACACGTGGGCG	40.0	42.9	55.1	56.8	122-126 bp	1.0	48.4
rpl36-infA_p3	TACAGAGGCACGTATTTTCA	TATGATTTAACACGTGGGCG	40.0	45.0	54.4	56.2	122-126 bp	1.0	48.4
rpl36-infA_p4	ACAGAGGCACGTATTTTCAT	CATATGATTTAACACGTGGGCG	40.0	45.5	55.1	58.1	123-127 bp	1.0	48.3
rpl36-infA_p5	ACAGAGGCACGTATTTTCAT	CCATATGATTTAACACGTGGGC	40.0	45.5	55.1	57.8	124-128 bp	1.0	48.3