Hotspot

ycf1

SSC · 127684-133122 · 5439 bp

Back to Brunsvigia

Pi 0.00558

MarkerSeek score 45.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5439
variable_sites: 56
pi: 0.00558
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCACAAATTCTGGGAAAAACCA	GAGACTTGGACCAATACGCA	40.9	50.0	58.2	57.6	223 bp	1.0	61.5
ycf1_p2	GCACAAATTCTGGGAAAAACCA	TGAGACTTGGACCAATACGC	40.9	50.0	58.2	57.6	224 bp	1.0	61.5
ycf1_p3	GCACAAATTCTGGGAAAAACC	GAGACTTGGACCAATACGCA	42.9	50.0	56.5	57.6	223 bp	1.0	59.0
ycf1_p4	GCACAAATTCTGGGAAAAACC	TGAGACTTGGACCAATACGC	42.9	50.0	56.5	57.6	224 bp	1.0	59.0
ycf1_p5	GGCACAAATTCTGGGAAAAAC	GAGACTTGGACCAATACGCA	42.9	50.0	56.5	57.6	224 bp	1.0	59.0