Hotspot

ndhD

SSC · 119070-120570 · 1501 bp

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Pi 0.0115

MarkerSeek score 56.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1501
variable_sites: 32
pi: 0.0115
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AAGACTTTTCTTTCTGTCTCA	CAAAAGCCCGTACTCGAGAA	33.3	50.0	52.6	57.9	1610-1618 bp	1.0	41.8
ndhD_p2	AAGACTTTTCTTTCTGTCTCA	CCTTTTTACCGGCAAAAGCC	33.3	50.0	52.6	58.2	1622-1630 bp	1.0	41.8
ndhD_p3	AAGACTTTTCTTTCTGTCTCA	CTTTTTACCGGCAAAAGCCC	33.3	50.0	52.6	58.2	1621-1629 bp	1.0	41.8
ndhD_p4	AGACTTTTCTTTCTGTCTCAT	CAAAAGCCCGTACTCGAGAA	33.3	50.0	52.4	57.9	1609-1617 bp	1.0	41.8
ndhD_p5	AGACTTTTCTTTCTGTCTCATT	CAAAAGCCCGTACTCGAGAA	31.8	50.0	53.3	57.9	1609-1617 bp	1.0	41.8