Hotspot

atpF

LSC · 12184-13622 · 1439 bp

Back to Brunsvigia

Pi 0.00973

MarkerSeek score 56.30

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1439
variable_sites: 25
pi: 0.00973
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TCTTTTCGGAAACAAAGGGA	AGTCCTATCTATAAGAGGAGAGCA	40.0	41.7	55.1	57.3	1515-1540 bp	0.75	31.9
atpF_p2	TCTTTTCGGAAACAAAGGGAA	AGTCCTATCTATAAGAGGAGAGCA	38.1	41.7	55.9	57.3	1515-1540 bp	0.75	31.9
atpF_p3	TTCTTTTCGGAAACAAAGGGA	AGTCCTATCTATAAGAGGAGAGCA	38.1	41.7	55.9	57.3	1516-1541 bp	0.75	31.9
atpF_p4	TCTTTTCGGAAACAAAGGGA	GTCCTATCTATAAGAGGAGAGCA	40.0	43.5	55.1	55.9	1514-1539 bp	0.75	31.9
atpF_p5	TCTTTTCGGAAACAAAGGGAAA	AGTCCTATCTATAAGAGGAGAGCA	36.4	41.7	56.7	57.3	1515-1540 bp	0.75	31.9