Hotspot

rpl2-trnH-GUG

IRa · 158339-158385 · 47 bp

Back to Brunsvigia

Pi 0.0426

MarkerSeek score 65.30

Primer available yes

Label rpl2-trnH-GUG

Feature Metadata

label_name: rpl2-trnH-GUG
feature_type: igs
region: IRa
length_bp: 47
variable_sites: 4
pi: 0.0426
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-trnH-GUG_p1	GTTATCCTGCGCTTGGAAGA	TGGATCAAGGCAGTGGATTG	50.0	50.0	58.0	57.9	183 bp	1.0	80.5
rpl2-trnH-GUG_p2	GTTATCCTGCGCTTGGAAGA	GGATCAAGGCAGTGGATTGT	50.0	50.0	58.0	57.9	182 bp	1.0	80.5
rpl2-trnH-GUG_p3	GTTATCCTGCGCTTGGAAGA	GTGGATCAAGGCAGTGGATT	50.0	50.0	58.0	57.9	184 bp	1.0	80.5
rpl2-trnH-GUG_p4	GTTATCCTGCGCTTGGAAGA	CGGATGTAGCCAAGTGGATC	50.0	55.0	58.0	58.1	197 bp	1.0	80.4
rpl2-trnH-GUG_p5	TATCCTGCGCTTGGAAGAAG	CGGATGTAGCCAAGTGGATC	50.0	55.0	57.7	58.1	195 bp	1.0	78.1