Hotspot

clpP-psbB

LSC · 74399-74831 · 433 bp

Back to Brunsvigia

Pi 0.0116

MarkerSeek score 63.60

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 433
variable_sites: 10
pi: 0.0116
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CCCAACCAGAAACTAGAGCC	50.0	55.0	58.0	57.9	608-609 bp	1.0	80.5
clpP-psbB_p2	CGCACTATACGTCAACCCAA	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.0	57.9	610-611 bp	1.0	80.5
clpP-psbB_p3	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	528-529 bp	1.0	79.8
clpP-psbB_p4	CGCACTATACGTCAACCCAA	CCAACCAGAAACTAGAGCCG	50.0	55.0	58.0	58.3	607-608 bp	1.0	79.3
clpP-psbB_p5	ACCCAAACTGCATCTTCCTC	CCCAACCAGAAACTAGAGCC	50.0	55.0	57.8	57.9	594-595 bp	1.0	78.9