Hotspot

ndhC-trnV-UAC

LSC · 51751-53465 · 1715 bp

Back to Brunsvigia

Pi 0.00671

MarkerSeek score 63.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1715
variable_sites: 22
pi: 0.00671
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1842-1873 bp	1.0	78.4
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCGGAAAT	AGCCCAATGCAACAAAATGC	50.0	45.0	57.9	58.5	1924-1955 bp	1.0	76.2
ndhC-trnV-UAC_p3	TCCTTCACTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	1855-1886 bp	1.0	75.8
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1841-1872 bp	1.0	75.7
ndhC-trnV-UAC_p5	CTGGTCCTTCACTAATCGGG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.4	57.8	1859-1890 bp	1.0	74.7