Hotspot

atpF-atpH

LSC · 13623-14119 · 497 bp

Back to Brunsvigia

Pi 0.0114

MarkerSeek score 64.30

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 497
variable_sites: 10
pi: 0.0114
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	ATTTATGGACTGGTCGTGGC	50.0	50.0	57.9	58.0	620-621 bp	1.0	80.0
atpF-atpH_p2	CAGTGGCCTAAGGAAACGAA	ATTTATGGACTGGTCGTGGC	50.0	50.0	57.8	58.0	592-593 bp	1.0	79.6
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	TCGTGGCATTAGCGCTTTTA	50.0	45.0	57.9	58.3	607-608 bp	1.0	78.2
atpF-atpH_p4	CAGTGGCCTAAGGAAACGAA	TCGTGGCATTAGCGCTTTTA	50.0	45.0	57.8	58.3	579-580 bp	1.0	77.8
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	CGTGGCATTAGCGCTTTTAT	50.0	45.0	57.9	57.2	606-607 bp	1.0	74.3