Hotspot

rps8

LSC · 83477-83881 · 405 bp

Back to Brunellia

Pi 0.00247

MarkerSeek score 42.00

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 405
variable_sites: 1
pi: 0.00247
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	TACGTCGGATCCTTCCTGAA	GGAAATCCAAAAGGAACGCG	50.0	50.0	57.9	58.0	968-975 bp	1.0	80.6
rps8_p2	TACGTCGGATCCTTCCTGAA	TCAGGGCTGTAATTGTACGC	50.0	50.0	57.9	58.0	1066-1073 bp	1.0	80.4
rps8_p3	TACATCGGGTCCTTTCGAGA	TCAGGGCTGTAATTGTACGC	50.0	50.0	57.9	58.0	1442-1449 bp	1.0	80.1
rps8_p4	AAAAACCCGCCCTTTCTCTT	TCAGGGCTGTAATTGTACGC	45.0	50.0	57.9	58.0	899 bp	0.5	59.2
rps8_p5	AAAAACCCGCCCTTTCTCTT	GGAAATCCAAAAGGAACGCG	45.0	50.0	57.9	58.0	801 bp	0.5	59.2