Hotspot

rps19

LSC · 87494-87772 · 279 bp

Back to Brunellia

Pi 0

MarkerSeek score 19.20

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 279
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 19.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TTGTCCCATAATCCCCCACT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.0	58.1	1045-1086 bp	1.0	83.5
rps19_p2	TTGTCCCATAATCCCCCACT	AACAGTCGGACAAGTAGGGA	50.0	50.0	58.0	58.0	1188-1229 bp	1.0	83.4
rps19_p3	TTGTCCCATAATCCCCCACT	ACAGTCGGACAAGTAGGGAA	50.0	50.0	58.0	58.0	1187-1228 bp	1.0	83.4
rps19_p4	TTGTCCCATAATCCCCCACT	AGTAGGGAATGTTGGGGTGA	50.0	50.0	58.0	57.9	1176-1217 bp	1.0	83.1
rps19_p5	TTGTCCCATAATCCCCCACT	CAACAGTCGGACAAGTAGGG	50.0	55.0	58.0	57.9	1189-1230 bp	1.0	82.8