Hotspot

ndhD

SSC · 118821-120317 · 1497 bp

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Pi 0.00401

MarkerSeek score 46.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1497
variable_sites: 6
pi: 0.00401
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GTAAATCCCGCAATTGTGGC	CAAATTGCTTCGGCTCCAAG	50.0	50.0	58.1	58.0	2162-2164 bp	1.0	79.9
ndhD_p2	GTAAATCCCGCAATTGTGGC	AAGATGTGAATCCGCCTGTC	50.0	50.0	58.1	58.0	2118-2120 bp	1.0	79.8
ndhD_p3	CTAATGAGGCATGGGGATCG	CAAATTGCTTCGGCTCCAAG	55.0	50.0	57.9	58.0	2278-2280 bp	1.0	79.8
ndhD_p4	CTAATGAGGCATGGGGATCG	AAGATGTGAATCCGCCTGTC	55.0	50.0	57.9	58.0	2234-2236 bp	1.0	79.6
ndhD_p5	TAAATCCCGCAATTGTGGCT	CAAATTGCTTCGGCTCCAAG	45.0	50.0	58.2	58.0	2161-2163 bp	1.0	79.3