Hotspot

ndhA

SSC · 122975-125169 · 2195 bp

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Pi 0.00547

MarkerSeek score 60.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2195
variable_sites: 12
pi: 0.00547
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TCCAGTTACCATAGGGAACA	GCCCACCGGGTTTTATCAAT	45.0	50.0	55.1	58.5	2417-2422 bp	1.0	53.3
ndhA_p2	ATCCAGTTACCATAGGGAACA	GCCCACCGGGTTTTATCAAT	42.9	50.0	55.7	58.5	2418-2423 bp	1.0	50.2
ndhA_p3	TCCAGTTACCATAGGGAACAT	GCCCACCGGGTTTTATCAAT	42.9	50.0	55.7	58.5	2417-2422 bp	1.0	50.2
ndhA_p4	GAATCCAGTTACCATAGGGAACA	GCCCACCGGGTTTTATCAAT	43.5	50.0	57.6	58.5	2420-2425 bp	1.0	49.3
ndhA_p5	TGAATCCAGTTACCATAGGGAAC	GCCCACCGGGTTTTATCAAT	43.5	50.0	57.6	58.5	2421-2426 bp	1.0	49.3