Hotspot

trnL-UAG-ccsA

SSC · 117499-117584 · 86 bp

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Pi 0.0123

MarkerSeek score 64.10

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 86
variable_sites: 1
pi: 0.0123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GTGAAATCGGTAGACACGCT	CAATTGAAACAGCCGAAAAGGA	50.0	40.9	58.0	58.0	232-237 bp	1.0	65.8
trnL-UAG-ccsA_p2	TGAAATCGGTAGACACGCTG	CAATTGAAACAGCCGAAAAGGA	50.0	40.9	58.0	58.0	231-236 bp	1.0	65.8
trnL-UAG-ccsA_p3	TAGACACGCTGCTCTTAGGA	CAATTGAAACAGCCGAAAAGGA	50.0	40.9	57.9	58.0	222-227 bp	1.0	65.0
trnL-UAG-ccsA_p4	GTGAAATCGGTAGACACGCT	ATTGAAACAGCCGAAAAGGA	50.0	40.0	58.0	55.9	230-235 bp	1.0	64.8
trnL-UAG-ccsA_p5	GTGAAATCGGTAGACACGCT	TTGAAACAGCCGAAAAGGAT	50.0	40.0	58.0	55.9	229-234 bp	1.0	64.8