Hotspot

rps19-rpl2

IRb · 87773-87842 · 70 bp

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Pi 0.0286

MarkerSeek score 68.90

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 70
variable_sites: 2
pi: 0.0286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	ATGGTAGATGTTCGGGACCA	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.1	58.1	308 bp	1.0	79.1
rps19-rpl2_p2	ATGGTAGATGTTCGGGACCA	TTGATTCTTCGTCGCCGTAG	50.0	50.0	58.1	58.1	216 bp	1.0	79.0
rps19-rpl2_p3	ATGGTAGATGTTCGGGACCA	TTGGGGTTATCCTGCACTTG	50.0	50.0	58.1	57.8	274 bp	1.0	77.9
rps19-rpl2_p4	GGTAGATGTTCGGGACCAAG	GGGCCCCAATTGGTAGAAAA	55.0	50.0	57.7	58.1	306 bp	1.0	77.6
rps19-rpl2_p5	ATGGTAGATGTTCGGGACCA	ATTTGATTCTTCGTCGCCGT	50.0	45.0	58.1	58.3	218 bp	1.0	77.5