Hotspot

rpl22-rps19

LSC · 87358-87493 · 136 bp

Back to Brunellia

Pi 0.0737

MarkerSeek score 65.00

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 136
variable_sites: 7
pi: 0.0737
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TTGTCCCATAATCCCCCACT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.0	58.1	1045-1086 bp	1.0	83.5
rpl22-rps19_p2	TTGTCCCATAATCCCCCACT	AACAGTCGGACAAGTAGGGA	50.0	50.0	58.0	58.0	1188-1229 bp	1.0	83.4
rpl22-rps19_p3	TTGTCCCATAATCCCCCACT	ACAGTCGGACAAGTAGGGAA	50.0	50.0	58.0	58.0	1187-1228 bp	1.0	83.4
rpl22-rps19_p4	TTGTCCCATAATCCCCCACT	AGTAGGGAATGTTGGGGTGA	50.0	50.0	58.0	57.9	1176-1217 bp	1.0	83.1
rpl22-rps19_p5	TTCCTTCGACACCAGAGGAT	ACAGTCGGACAAGTAGGGAA	50.0	50.0	58.1	58.0	1356-1397 bp	1.0	82.5