Hotspot

ycf3-trnS-GGA

LSC · 47112-48003 · 892 bp

Back to Brunellia

Pi 0.00675

MarkerSeek score 63.00

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 892
variable_sites: 6
pi: 0.00675
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	1052-1055 bp	1.0	67.3
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	1053-1056 bp	1.0	67.3
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	1073-1076 bp	1.0	66.8
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	1074-1077 bp	1.0	66.8
ycf3-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	TGGGTGAAGGTACGGAAAGA	45.0	50.0	56.4	58.3	1084-1087 bp	1.0	65.7