Hotspot

trnK-UUU-rps16

LSC · 4647-5958 · 1312 bp

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Pi 0.00864
MarkerSeek score 66.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1312
variable_sites
10
pi
0.00864
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 1322-1439 bp 1.0 81.7
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 1321-1438 bp 1.0 81.7
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TAAGGCGGGGGTTTTTACAG 50.0 50.0 58.2 57.8 1284-1401 bp 1.0 81.6
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TAAGGCGGGGGTTTTTACAG 50.0 50.0 58.2 57.8 1283-1400 bp 1.0 81.6
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA GGTGCTCAACCTACAGGAAC 50.0 55.0 58.3 58.2 1331-1448 bp 1.0 80.8