Hotspot

trnS-UGA

LSC · 35583-35671 · 89 bp

Back to Bruinsmia

Pi 0.0337

MarkerSeek score 69.00

Primer available yes

Label trnS-UGA

Feature Metadata

label_name: trnS-UGA
feature_type: tRNA
region: LSC
length_bp: 89
variable_sites: 3
pi: 0.0337
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA_p1	TTTTCTGGCTTGGCTAGGTG	TCGACCTCTAGATTCACACCT	50.0	47.6	58.1	57.6	298 bp	1.0	69.4
trnS-UGA_p2	GATAGCCGAGCCATTTTCCT	TCGACCTCTAGATTCACACCT	50.0	47.6	57.7	57.6	277 bp	1.0	68.1
trnS-UGA_p3	CCGGTCCGGTAAAAACCAAT	TCGACCTCTAGATTCACACCT	50.0	47.6	58.5	57.6	245 bp	1.0	66.7
trnS-UGA_p4	TAGCCGAGCCATTTTCCTTT	TCGACCTCTAGATTCACACCT	45.0	47.6	57.5	57.6	275 bp	1.0	66.2
trnS-UGA_p5	TTTTCTGGCTTGGCTAGGTG	CGATAGGGGTGGTATTCACT	50.0	50.0	58.1	56.1	264 bp	1.0	65.1