Hotspot

trnF-GAA

LSC · 48689-48761 · 73 bp

Back to Bruinsmia

Pi 0.0548

MarkerSeek score 67.60

Primer available yes

Label trnF-GAA

Feature Metadata

label_name: trnF-GAA
feature_type: tRNA
region: LSC
length_bp: 73
variable_sites: 4
pi: 0.0548
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA_p1	ACGACCCCAATCTGTATCCT	TTGTCTTATGGTACGCGTGG	50.0	50.0	57.8	58.0	1345-1355 bp	1.0	80.8
trnF-GAA_p2	ACGACCCCAATCTGTATCCT	AGTCATGTGGGAGGGAATGA	50.0	50.0	57.8	58.0	1170-1179 bp	1.0	80.7
trnF-GAA_p3	ACGACCCCAATCTGTATCCT	AGAAAGTCATGTGGGAGGGA	50.0	50.0	57.8	58.0	1174-1183 bp	1.0	80.5
trnF-GAA_p4	TGACTGTGTCGGTAGAGGAA	TTGTCTTATGGTACGCGTGG	50.0	50.0	57.7	58.0	1445-1455 bp	1.0	79.9
trnF-GAA_p5	ACGACCCCAATCTGTATCCT	GCGTGGTCCAACAACAAAAA	50.0	45.0	57.8	58.0	1341 bp	0.5	58.6