Hotspot

trnL-UAG-ccsA

SSC · 117358-117469 · 112 bp

Back to Bruinsmia

Pi 0.116

MarkerSeek score 73.00

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 112
variable_sites: 13
pi: 0.116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	CCCGCTTTCAACCGAATCTA	CAACCACTCGGATTGGAGAG	50.0	55.0	58.0	58.0	1044-1045 bp	1.0	82.0
trnL-UAG-ccsA_p2	CCCGCTTTCAACCGAATCTA	ACCCGAAGTAGTAAAGCCCT	50.0	50.0	58.0	58.1	984-985 bp	1.0	81.9
trnL-UAG-ccsA_p3	AACAAGCCGCTATGGTGAAA	CAACCACTCGGATTGGAGAG	45.0	55.0	58.1	58.0	605-607 bp	1.0	81.4
trnL-UAG-ccsA_p4	TTATGCAGTGCGAATTCGGA	CAACCACTCGGATTGGAGAG	45.0	55.0	58.0	58.0	1256 bp	0.5	59.7
trnL-UAG-ccsA_p5	TTATGCAGTGCGAATTCGGA	ACCCGAAGTAGTAAAGCCCT	45.0	50.0	58.0	58.1	1196 bp	0.5	59.5