Hotspot

infA-rps8

LSC · 82400-82520 · 121 bp

Back to Bruinsmia

Pi 0.0579

MarkerSeek score 67.50

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 121
variable_sites: 7
pi: 0.0579
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	CGAACCCGGAACATACCATT	GAACTCGAGTTTTTGGTGCG	50.0	50.0	58.0	58.0	913-924 bp	1.0	83.8
infA-rps8_p2	AGAAACCACCCGACCTCTTA	GAACTCGAGTTTTTGGTGCG	50.0	50.0	58.0	58.0	1571-1612 bp	1.0	82.6
infA-rps8_p3	CGTGAACCAATACGTCCACT	GAACTCGAGTTTTTGGTGCG	50.0	50.0	57.9	58.0	1471-1512 bp	1.0	82.3
infA-rps8_p4	CGAACCCGGAACATACCATT	GAAGGAATCGGCGGAGAAAT	50.0	50.0	58.0	58.0	237-278 bp	1.0	82.2
infA-rps8_p5	AGAAACCACCCGACCTCTTA	GAAGGAATCGGCGGAGAAAT	50.0	50.0	58.0	58.0	895-966 bp	1.0	81.0