Hotspot

rps12-psbB

LSC · 72485-74716 · 2232 bp

Back to Bruinsmia

Pi 0.055

MarkerSeek score 85.80

Primer available yes

Label rps12-psbB

Feature Metadata

label_name: rps12-psbB
feature_type: igs
region: LSC
length_bp: 2232
variable_sites: 115
pi: 0.055
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 85.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-psbB_p1	GCGGGAGATTTCGTGACATT	CGATACCAAGGCAAACCCAT	50.0	50.0	58.6	58.2	2231-2320 bp	1.0	81.1
rps12-psbB_p2	GAAGAGCGGGAGATTTCGTG	CGATACCAAGGCAAACCCAT	55.0	50.0	58.7	58.2	2236-2325 bp	1.0	80.4
rps12-psbB_p3	GCGGGAGATTTCGTGACATT	ATACAGAGCCATTGAACCGG	50.0	50.0	58.6	57.7	2331-2420 bp	1.0	80.2
rps12-psbB_p4	GTACATGTTCCTCGACGCTG	CGATACCAAGGCAAACCCAT	55.0	50.0	58.7	58.2	2267-2356 bp	1.0	80.2
rps12-psbB_p5	GCGGGAGATTTCGTGACATT	AGCAACCAAAGCTGTATGCA	50.0	45.0	58.6	58.4	2304-2393 bp	1.0	79.8