Hotspot

rbcL-accD

LSC · 57236-59846 · 2611 bp

Back to Bruinsmia

Pi 0.121

MarkerSeek score 84.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 2611
variable_sites: 276
pi: 0.121
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	AGCATACTCGATCAAACGGG	55.0	50.0	58.0	57.8	2757-2914 bp	1.0	89.8
rbcL-accD_p2	AATGAGGGACGTGATCTTGC	AGCATACTCGATCAAACGGG	50.0	50.0	58.0	57.8	2802-2959 bp	1.0	89.7
rbcL-accD_p3	GCTGCTTGTGAGGTATGGAA	AGCATACTCGATCAAACGGG	50.0	50.0	57.9	57.8	2727-2884 bp	1.0	89.2
rbcL-accD_p4	AATGAGGGACGTGATCTTGC	ATAACCCCAATTGCTACGGG	50.0	50.0	58.0	57.6	2729-2886 bp	1.0	88.9
rbcL-accD_p5	TGATCTTGCTCGTGAGGGTA	AGCATACTCGATCAAACGGG	50.0	50.0	58.2	57.8	2791-2948 bp	1.0	88.8