Hotspot

atpH-atpF

LSC · 20922-21288 · 367 bp

Back to Bruinsmia

Pi 0.0436

MarkerSeek score 73.10

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 367
variable_sites: 16
pi: 0.0436
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	GGCTGGTTGTAGCATTAGCA	CCAGTAGCCCAAAGAAACGA	50.0	50.0	58.2	57.8	456-471 bp	1.0	79.3
atpH-atpF_p2	TGGGCTGGTTGTAGCATTAG	CCAGTAGCCCAAAGAAACGA	50.0	50.0	57.6	57.8	458-473 bp	1.0	78.0
atpH-atpF_p3	GGCTGGTTGTAGCATTAGCA	TATTAAGCCCGAAACTCCCG	50.0	50.0	58.2	57.4	484-499 bp	1.0	75.7
atpH-atpF_p4	CGGAGGGAAAAATACGAGGT	CCAGTAGCCCAAAGAAACGA	50.0	50.0	57.3	57.8	522-537 bp	1.0	75.5
atpH-atpF_p5	GCGGAGGGAAAAATACGAGG	CCAGTAGCCCAAAGAAACGA	55.0	50.0	58.7	57.8	523-538 bp	1.0	75.4